Plasmid_Backbone

Part:BBa_J72114

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-09)

Complete, arabinose inducible phagemid, CmR, p15a vector

Vector contains all of the cis elements required to be an efficient phagemid. Arabinose induces lysis. Part is referred to internally as pBjk3019.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6579
    Illegal NheI site found at 920
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BglII site found at 6570
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6579
    Illegal AgeI site found at 1006
    Illegal AgeI site found at 1330
    Illegal AgeI site found at 4096
    Illegal AgeI site found at 5542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 4724
    Illegal SapI.rc site found at 5559


[edit]
Categories
Parameters
None